Saline:Article Title: Overcoming ovarian cancer resistance and evasion to CAR-T cell therapy by harnessing allogeneic CAR-NKT cells.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Lenti/FG This paper N/A Lenti/CD1d This paper N/A Lenti/MSLN This paper N/A Lenti/STAT1C-EGFP This paper N/A Biological samples Human peripheral blood mononuclear cells (PBMCs) UCLA N/A Primary ovarian cancer (OC) patient samples UCLA N/A Cord Blood CD34+ Stem/Progenitor Cells HemaCare (Charles River) CAT#CB34C-3 Chemicals, peptides, and recombinant proteins Streptavidin-HRP conjugate Invitrogen CAT#SA10001 IFN-γ (ELISA, standard) eBioscience CAT#29-8319-65 TNFα (ELISA, standard) eBioscience CAT#29-8329-65 IL-2 (ELISA, standard) eBioscience CAT#29-8029-65 IL-4 (ELISA, standard) eBioscience CAT#39-8049-65 IL-17A (ELISA, standard) eBioscience CAT#29-8179-65 Tetramethylbenzidine (TMB) KPL CAT#5120-0053 Ganciclovir (GCV) Sigma CAT#G2536 α-Galactosylceramide (KRN7000) Avanti Polar Lipids SKU#867000P-1mg Recombinant human IL-2 Peprotech CAT#200-02 Recombinant human IL-3 Peprotech CAT#200-03 Recombinant human IL-4 Peprotech CAT#200-04 Recombinant human IL-7 Peprotech CAT#200-07 Recombinant human IL-13 Peprotech CAT#200-13 Recombinant human IL-15 Peprotech CAT#200-15 Recombinant human IFN-γ Peprotech CAT#300-02 Recombinant human Flt3-Ligand Peprotech CAT#300-19 Recombinant human SCF Peprotech CAT#300-07 Recombinant human TPO Peprotech CAT#300-18 Recombinant human GM-CSF Peprotech CAT#300-03 Recombinant human M-CSF Peprotech CAT#300-25 L-ascorbic acid 2-phosphate Sigma CAT#A8960-5G B27TM Supplement (50X), serum free ThermoFisher CAT#17504044 Cas9-NLS purified protein UC Berkeley N/A X-VIVO 15 Serum-free Hematopoietic Cell Medium Lonza CAT#04-418Q RPMI1640 cell culture medium Corning Cellgro CAT#10-040-CV DMEM cell culture medium Corning Cellgro CAT#10-013-CV Fetal Bovine Serum (FBS) Sigma CAT#F2442 MACS BSA stock solution Miltenyi CAT#130-091-376 30% BSA Gemini CAT#700-110-100 Penicillin-StreptomycineGlutamine (P/S/G) Gibco CAT#10378016 Penicillin: streptomycin (pen:strep) solution (P/S) Gemini Bio-products CAT#400-109-100 MEM non-essential amino acids (NEAA) Gibco CAT#11140050 HEPES Buffer Solution Gibco CAT#15630080 Sodium Pyruvate Gibco CAT#11360070 Beta-Mercaptoethanol Sigma SKU#M6250 (Continued on next page) e3 Med 6, 100804, October 10, 2025 .. REAGENT or RESOURCE SOURCE IDENTIFIER Normocin Invivogen CATant-nr-2 Fixable Viability Dye eFluor506 eBioscience CAT#65-0866-14 Cell Fixation/Permeabilization Kit BD Biosciences CAT#554714 RetroNectin recombination human fibronectin fragment, 2.5mg Takara CAT#T100B 10% neutral-buffered formalin Richard-Allan Scientific CAT#5705 D-Luciferin Potassium Salt Bioluminescent Substrate Revivi CAT#122799-100MG Fluriso (Isoflurane) Vet One CAT#502017 Phosphate Buffered Saline (PBS) pH 7.4 (1X) Gibco CAT#10010-023 Formaldehyde Sigma-Aldrich CAT#F8775 Golgistop Protein Transport Inhibitor BD Biosciences CAT#554724 Poloxamer Synperonic F108 Millipore Sigma CAT#07579-250G-F Prostaglandin E2 (PGE2) Cayman Chemical CAT#14010 TrypLETM Express Enzyme ThermoFisher Scientific CAT#12605010 Phorbol-12-myristate-13-acetate (PMA) Sigma CAT#524400 Ionomycin, Calcium salt, Streptomyces conglobatus Sigma CAT#407952 Critical commercial assays Human NK Cell Isolation Kit Miltenyi Biotec CAT#130-092-657 Human CD34 MicroBeads Kit Miltenyi Biotec CAT#130-046-703 Human CD14 MicroBeads Kit Miltenyi Biotec CAT#130-050-201 Human Anti-NKT MicroBeads Miltenyi Biotec CAT#130-094-842 Human Anti-HLA-DR MicroBeads Miltenyi Biotec CAT#130-046-101 Human tumor cell isolation kit Miltenyi Biotec CAT#130-108-339 Fixation/Permeabilization Solution Kit BD Sciences CAT#55474 AmaxaTM P3 Primary Cell 4DNucleofectorTM X Kit S Lonza CAT#V4XP-3032 Dynabeads Human T-Activator CD3/CD28 ThermoFisher CAT#11131D miRNeasy Mini Kit Qiagen CAT#217004 Chromium single cell V(D)J enrichment kit, human T cell 10 x Genomics CAT#1000005 Cryostor cell cryopreservation media Sigma CAT#C2874-100ML StemSpan"" Lymphoid Differentiation Coating Material (100X) Stem Cell Technologies CAT#9925 StemSpan" SFEM II Stem Cell Technologies CAT#9605 ImmunoCult" Human CD3/CD28/CD2 T Cell Activator Stem Cell Technologies CAT#10970 TransIT-Lenti Transfection Reagent Mirus Bio CAT#MIR 6600 Amicon Ultra-15 Centrifugal Filter Unit Sigma CAT#UFC910024 Nunclon Sphera-Treated 96-Well Microplate ThermoFisher CAT#174929 CellQART 6-Well Cell Culture Insert Millicell CAT#9301002 Human IL-17A ELISA MAX Deluxe Kit Biolegend CAT#433915 Deposited data scRNA-seq for the primary OC patient sample profiling study This paper GEO: GSE272764 scRNA-seq for the in vivo therapeutic cell profiling study This paper GEO: GSE270820 (Continued on next page) Med 6, 100804, October 10, 2025 e4 .. REAGENT or RESOURCE SOURCE IDENTIFIER scRNA-seq for the in vivo tumor cell profiling study This paper GEO: GSE255431 scRNA-seq for the in vivo TME profiling study This paper GEO: GSE268749 scTCR-seq This paper GEO: GSE254262 Bulk RNA-seq (dataset 1) This paper GEO: GSE252379 Bulk RNA-seq (dataset 2) This paper GEO: GSE164425 Methyl-seq This paper GEO: GSE251909 scRNA-seq for primary OC patient sample profiling study (public dataset B) Zhang et al.50 GEO: GSE165897 scRNA-seq for primary OC patient sample profiling study (public dataset C) Xu et al.49 GEO: GSE184800 scRNA-seq for primary OC patient sample profiling study (public dataset D) Zheng et al.36 GSA-Human, PRJCA005422 Experimental models: Cell lines Human multiple myeloma (MM) cell line MM.1S ATCC CRL-2974 Human chronic myelogenous leukemia cancer cell line K562 ATCC CCL-243 Human ovarian cancer cell line OVCAR3 ATCC HTB-161 Human ovarian cancer cell line OVCAR8 NIH N/A Human ovarian cancer cell line SKOV3 ATCC HTB-77 Human multiple myeloma (MM) cell line MM.1S-FG This paper N/A Human multiple myeloma (MM) cell line MM.1S-MSLN-FG This paper N/A Human ovarian cancer cell line OVCAR3-FG This paper N/A Human ovarian cancer cell line OVCAR3-FGMSLN-/This paper N/A Human ovarian cancer cell line OVCAR8-FG This paper N/A Human ovarian cancer cell line SKOV3-FG This paper N/A Human artificial APC cell line This paper N/A Experimental models: Organisms/strains NOD.Cg-Prkdcscid Il2rgtm1Wjl/SzJ (NSG) The Jackson Laboratory Stock #: 005557 Oligonucleotides gRNA (MSLN): GCCAACGGCTCGAC CCCTGT Synthego N/A Recombinant DNA Vector: parental lentivector pMNDW Giannoni et al.67; Lan et al.171 N/A Software and algorithms FlowJo Software FlowJo https://www.flowjo.com/solutions/flowjo/ downloads AURA imaging software Spectral Instruments Imaging https://spectralinvivo.com/software/ ImageJ ImageJ https://imagej.net/Downloads Prism 9 Graphpad https://www.graphpad.com/scientific- software/prism/ Matlab The MathWorks, Inc http://www.mathworks.com/products/ matlab.html R R http://www.R-project.org/ RStudio RStudio https://posit.co e5 Med 6, 100804, October 10, 2025
Cell Isolation:Article Title: Overcoming ovarian cancer resistance and evasion to CAR-T cell therapy by harnessing allogeneic CAR-NKT cells.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Lenti/FG This paper N/A Lenti/CD1d This paper N/A Lenti/MSLN This paper N/A Lenti/STAT1C-EGFP This paper N/A Biological samples Human peripheral blood mononuclear cells (PBMCs) UCLA N/A Primary ovarian cancer (OC) patient samples UCLA N/A Cord Blood CD34+ Stem/Progenitor Cells HemaCare (Charles River) CAT#CB34C-3 Chemicals, peptides, and recombinant proteins Streptavidin-HRP conjugate Invitrogen CAT#SA10001 IFN-γ (ELISA, standard) eBioscience CAT#29-8319-65 TNFα (ELISA, standard) eBioscience CAT#29-8329-65 IL-2 (ELISA, standard) eBioscience CAT#29-8029-65 IL-4 (ELISA, standard) eBioscience CAT#39-8049-65 IL-17A (ELISA, standard) eBioscience CAT#29-8179-65 Tetramethylbenzidine (TMB) KPL CAT#5120-0053 Ganciclovir (GCV) Sigma CAT#G2536 α-Galactosylceramide (KRN7000) Avanti Polar Lipids SKU#867000P-1mg Recombinant human IL-2 Peprotech CAT#200-02 Recombinant human IL-3 Peprotech CAT#200-03 Recombinant human IL-4 Peprotech CAT#200-04 Recombinant human IL-7 Peprotech CAT#200-07 Recombinant human IL-13 Peprotech CAT#200-13 Recombinant human IL-15 Peprotech CAT#200-15 Recombinant human IFN-γ Peprotech CAT#300-02 Recombinant human Flt3-Ligand Peprotech CAT#300-19 Recombinant human SCF Peprotech CAT#300-07 Recombinant human TPO Peprotech CAT#300-18 Recombinant human GM-CSF Peprotech CAT#300-03 Recombinant human M-CSF Peprotech CAT#300-25 L-ascorbic acid 2-phosphate Sigma CAT#A8960-5G B27TM Supplement (50X), serum free ThermoFisher CAT#17504044 Cas9-NLS purified protein UC Berkeley N/A X-VIVO 15 Serum-free Hematopoietic Cell Medium Lonza CAT#04-418Q RPMI1640 cell culture medium Corning Cellgro CAT#10-040-CV DMEM cell culture medium Corning Cellgro CAT#10-013-CV Fetal Bovine Serum (FBS) Sigma CAT#F2442 MACS BSA stock solution Miltenyi CAT#130-091-376 30% BSA Gemini CAT#700-110-100 Penicillin-StreptomycineGlutamine (P/S/G) Gibco CAT#10378016 Penicillin: streptomycin (pen:strep) solution (P/S) Gemini Bio-products CAT#400-109-100 MEM non-essential amino acids (NEAA) Gibco CAT#11140050 HEPES Buffer Solution Gibco CAT#15630080 Sodium Pyruvate Gibco CAT#11360070 Beta-Mercaptoethanol Sigma SKU#M6250 (Continued on next page) e3 Med 6, 100804, October 10, 2025 .. REAGENT or RESOURCE SOURCE IDENTIFIER Normocin Invivogen CATant-nr-2 Fixable Viability Dye eFluor506 eBioscience CAT#65-0866-14 Cell Fixation/Permeabilization Kit BD Biosciences CAT#554714 RetroNectin recombination human fibronectin fragment, 2.5mg Takara CAT#T100B 10% neutral-buffered formalin Richard-Allan Scientific CAT#5705 D-Luciferin Potassium Salt Bioluminescent Substrate Revivi CAT#122799-100MG Fluriso (Isoflurane) Vet One CAT#502017 Phosphate Buffered Saline (PBS) pH 7.4 (1X) Gibco CAT#10010-023 Formaldehyde Sigma-Aldrich CAT#F8775 Golgistop Protein Transport Inhibitor BD Biosciences CAT#554724 Poloxamer Synperonic F108 Millipore Sigma CAT#07579-250G-F Prostaglandin E2 (PGE2) Cayman Chemical CAT#14010 TrypLETM Express Enzyme ThermoFisher Scientific CAT#12605010 Phorbol-12-myristate-13-acetate (PMA) Sigma CAT#524400 Ionomycin, Calcium salt, Streptomyces conglobatus Sigma CAT#407952 Critical commercial assays Human NK Cell Isolation Kit Miltenyi Biotec CAT#130-092-657 Human CD34 MicroBeads Kit Miltenyi Biotec CAT#130-046-703 Human CD14 MicroBeads Kit Miltenyi Biotec CAT#130-050-201 Human Anti-NKT MicroBeads Miltenyi Biotec CAT#130-094-842 Human Anti-HLA-DR MicroBeads Miltenyi Biotec CAT#130-046-101 Human tumor cell isolation kit Miltenyi Biotec CAT#130-108-339 Fixation/Permeabilization Solution Kit BD Sciences CAT#55474 AmaxaTM P3 Primary Cell 4DNucleofectorTM X Kit S Lonza CAT#V4XP-3032 Dynabeads Human T-Activator CD3/CD28 ThermoFisher CAT#11131D miRNeasy Mini Kit Qiagen CAT#217004 Chromium single cell V(D)J enrichment kit, human T cell 10 x Genomics CAT#1000005 Cryostor cell cryopreservation media Sigma CAT#C2874-100ML StemSpan"" Lymphoid Differentiation Coating Material (100X) Stem Cell Technologies CAT#9925 StemSpan" SFEM II Stem Cell Technologies CAT#9605 ImmunoCult" Human CD3/CD28/CD2 T Cell Activator Stem Cell Technologies CAT#10970 TransIT-Lenti Transfection Reagent Mirus Bio CAT#MIR 6600 Amicon Ultra-15 Centrifugal Filter Unit Sigma CAT#UFC910024 Nunclon Sphera-Treated 96-Well Microplate ThermoFisher CAT#174929 CellQART 6-Well Cell Culture Insert Millicell CAT#9301002 Human IL-17A ELISA MAX Deluxe Kit Biolegend CAT#433915 Deposited data scRNA-seq for the primary OC patient sample profiling study This paper GEO: GSE272764 scRNA-seq for the in vivo therapeutic cell profiling study This paper GEO: GSE270820 (Continued on next page) Med 6, 100804, October 10, 2025 e4 .. REAGENT or RESOURCE SOURCE IDENTIFIER scRNA-seq for the in vivo tumor cell profiling study This paper GEO: GSE255431 scRNA-seq for the in vivo TME profiling study This paper GEO: GSE268749 scTCR-seq This paper GEO: GSE254262 Bulk RNA-seq (dataset 1) This paper GEO: GSE252379 Bulk RNA-seq (dataset 2) This paper GEO: GSE164425 Methyl-seq This paper GEO: GSE251909 scRNA-seq for primary OC patient sample profiling study (public dataset B) Zhang et al.50 GEO: GSE165897 scRNA-seq for primary OC patient sample profiling study (public dataset C) Xu et al.49 GEO: GSE184800 scRNA-seq for primary OC patient sample profiling study (public dataset D) Zheng et al.36 GSA-Human, PRJCA005422 Experimental models: Cell lines Human multiple myeloma (MM) cell line MM.1S ATCC CRL-2974 Human chronic myelogenous leukemia cancer cell line K562 ATCC CCL-243 Human ovarian cancer cell line OVCAR3 ATCC HTB-161 Human ovarian cancer cell line OVCAR8 NIH N/A Human ovarian cancer cell line SKOV3 ATCC HTB-77 Human multiple myeloma (MM) cell line MM.1S-FG This paper N/A Human multiple myeloma (MM) cell line MM.1S-MSLN-FG This paper N/A Human ovarian cancer cell line OVCAR3-FG This paper N/A Human ovarian cancer cell line OVCAR3-FGMSLN-/This paper N/A Human ovarian cancer cell line OVCAR8-FG This paper N/A Human ovarian cancer cell line SKOV3-FG This paper N/A Human artificial APC cell line This paper N/A Experimental models: Organisms/strains NOD.Cg-Prkdcscid Il2rgtm1Wjl/SzJ (NSG) The Jackson Laboratory Stock #: 005557 Oligonucleotides gRNA (MSLN): GCCAACGGCTCGAC CCCTGT Synthego N/A Recombinant DNA Vector: parental lentivector pMNDW Giannoni et al.67; Lan et al.171 N/A Software and algorithms FlowJo Software FlowJo https://www.flowjo.com/solutions/flowjo/ downloads AURA imaging software Spectral Instruments Imaging https://spectralinvivo.com/software/ ImageJ ImageJ https://imagej.net/Downloads Prism 9 Graphpad https://www.graphpad.com/scientific- software/prism/ Matlab The MathWorks, Inc http://www.mathworks.com/products/ matlab.html R R http://www.R-project.org/ RStudio RStudio https://posit.co e5 Med 6, 100804, October 10, 2025
Single Cell:Article Title: Overcoming ovarian cancer resistance and evasion to CAR-T cell therapy by harnessing allogeneic CAR-NKT cells.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Lenti/FG This paper N/A Lenti/CD1d This paper N/A Lenti/MSLN This paper N/A Lenti/STAT1C-EGFP This paper N/A Biological samples Human peripheral blood mononuclear cells (PBMCs) UCLA N/A Primary ovarian cancer (OC) patient samples UCLA N/A Cord Blood CD34+ Stem/Progenitor Cells HemaCare (Charles River) CAT#CB34C-3 Chemicals, peptides, and recombinant proteins Streptavidin-HRP conjugate Invitrogen CAT#SA10001 IFN-γ (ELISA, standard) eBioscience CAT#29-8319-65 TNFα (ELISA, standard) eBioscience CAT#29-8329-65 IL-2 (ELISA, standard) eBioscience CAT#29-8029-65 IL-4 (ELISA, standard) eBioscience CAT#39-8049-65 IL-17A (ELISA, standard) eBioscience CAT#29-8179-65 Tetramethylbenzidine (TMB) KPL CAT#5120-0053 Ganciclovir (GCV) Sigma CAT#G2536 α-Galactosylceramide (KRN7000) Avanti Polar Lipids SKU#867000P-1mg Recombinant human IL-2 Peprotech CAT#200-02 Recombinant human IL-3 Peprotech CAT#200-03 Recombinant human IL-4 Peprotech CAT#200-04 Recombinant human IL-7 Peprotech CAT#200-07 Recombinant human IL-13 Peprotech CAT#200-13 Recombinant human IL-15 Peprotech CAT#200-15 Recombinant human IFN-γ Peprotech CAT#300-02 Recombinant human Flt3-Ligand Peprotech CAT#300-19 Recombinant human SCF Peprotech CAT#300-07 Recombinant human TPO Peprotech CAT#300-18 Recombinant human GM-CSF Peprotech CAT#300-03 Recombinant human M-CSF Peprotech CAT#300-25 L-ascorbic acid 2-phosphate Sigma CAT#A8960-5G B27TM Supplement (50X), serum free ThermoFisher CAT#17504044 Cas9-NLS purified protein UC Berkeley N/A X-VIVO 15 Serum-free Hematopoietic Cell Medium Lonza CAT#04-418Q RPMI1640 cell culture medium Corning Cellgro CAT#10-040-CV DMEM cell culture medium Corning Cellgro CAT#10-013-CV Fetal Bovine Serum (FBS) Sigma CAT#F2442 MACS BSA stock solution Miltenyi CAT#130-091-376 30% BSA Gemini CAT#700-110-100 Penicillin-StreptomycineGlutamine (P/S/G) Gibco CAT#10378016 Penicillin: streptomycin (pen:strep) solution (P/S) Gemini Bio-products CAT#400-109-100 MEM non-essential amino acids (NEAA) Gibco CAT#11140050 HEPES Buffer Solution Gibco CAT#15630080 Sodium Pyruvate Gibco CAT#11360070 Beta-Mercaptoethanol Sigma SKU#M6250 (Continued on next page) e3 Med 6, 100804, October 10, 2025 .. REAGENT or RESOURCE SOURCE IDENTIFIER Normocin Invivogen CATant-nr-2 Fixable Viability Dye eFluor506 eBioscience CAT#65-0866-14 Cell Fixation/Permeabilization Kit BD Biosciences CAT#554714 RetroNectin recombination human fibronectin fragment, 2.5mg Takara CAT#T100B 10% neutral-buffered formalin Richard-Allan Scientific CAT#5705 D-Luciferin Potassium Salt Bioluminescent Substrate Revivi CAT#122799-100MG Fluriso (Isoflurane) Vet One CAT#502017 Phosphate Buffered Saline (PBS) pH 7.4 (1X) Gibco CAT#10010-023 Formaldehyde Sigma-Aldrich CAT#F8775 Golgistop Protein Transport Inhibitor BD Biosciences CAT#554724 Poloxamer Synperonic F108 Millipore Sigma CAT#07579-250G-F Prostaglandin E2 (PGE2) Cayman Chemical CAT#14010 TrypLETM Express Enzyme ThermoFisher Scientific CAT#12605010 Phorbol-12-myristate-13-acetate (PMA) Sigma CAT#524400 Ionomycin, Calcium salt, Streptomyces conglobatus Sigma CAT#407952 Critical commercial assays Human NK Cell Isolation Kit Miltenyi Biotec CAT#130-092-657 Human CD34 MicroBeads Kit Miltenyi Biotec CAT#130-046-703 Human CD14 MicroBeads Kit Miltenyi Biotec CAT#130-050-201 Human Anti-NKT MicroBeads Miltenyi Biotec CAT#130-094-842 Human Anti-HLA-DR MicroBeads Miltenyi Biotec CAT#130-046-101 Human tumor cell isolation kit Miltenyi Biotec CAT#130-108-339 Fixation/Permeabilization Solution Kit BD Sciences CAT#55474 AmaxaTM P3 Primary Cell 4DNucleofectorTM X Kit S Lonza CAT#V4XP-3032 Dynabeads Human T-Activator CD3/CD28 ThermoFisher CAT#11131D miRNeasy Mini Kit Qiagen CAT#217004 Chromium single cell V(D)J enrichment kit, human T cell 10 x Genomics CAT#1000005 Cryostor cell cryopreservation media Sigma CAT#C2874-100ML StemSpan"" Lymphoid Differentiation Coating Material (100X) Stem Cell Technologies CAT#9925 StemSpan" SFEM II Stem Cell Technologies CAT#9605 ImmunoCult" Human CD3/CD28/CD2 T Cell Activator Stem Cell Technologies CAT#10970 TransIT-Lenti Transfection Reagent Mirus Bio CAT#MIR 6600 Amicon Ultra-15 Centrifugal Filter Unit Sigma CAT#UFC910024 Nunclon Sphera-Treated 96-Well Microplate ThermoFisher CAT#174929 CellQART 6-Well Cell Culture Insert Millicell CAT#9301002 Human IL-17A ELISA MAX Deluxe Kit Biolegend CAT#433915 Deposited data scRNA-seq for the primary OC patient sample profiling study This paper GEO: GSE272764 scRNA-seq for the in vivo therapeutic cell profiling study This paper GEO: GSE270820 (Continued on next page) Med 6, 100804, October 10, 2025 e4 .. REAGENT or RESOURCE SOURCE IDENTIFIER scRNA-seq for the in vivo tumor cell profiling study This paper GEO: GSE255431 scRNA-seq for the in vivo TME profiling study This paper GEO: GSE268749 scTCR-seq This paper GEO: GSE254262 Bulk RNA-seq (dataset 1) This paper GEO: GSE252379 Bulk RNA-seq (dataset 2) This paper GEO: GSE164425 Methyl-seq This paper GEO: GSE251909 scRNA-seq for primary OC patient sample profiling study (public dataset B) Zhang et al.50 GEO: GSE165897 scRNA-seq for primary OC patient sample profiling study (public dataset C) Xu et al.49 GEO: GSE184800 scRNA-seq for primary OC patient sample profiling study (public dataset D) Zheng et al.36 GSA-Human, PRJCA005422 Experimental models: Cell lines Human multiple myeloma (MM) cell line MM.1S ATCC CRL-2974 Human chronic myelogenous leukemia cancer cell line K562 ATCC CCL-243 Human ovarian cancer cell line OVCAR3 ATCC HTB-161 Human ovarian cancer cell line OVCAR8 NIH N/A Human ovarian cancer cell line SKOV3 ATCC HTB-77 Human multiple myeloma (MM) cell line MM.1S-FG This paper N/A Human multiple myeloma (MM) cell line MM.1S-MSLN-FG This paper N/A Human ovarian cancer cell line OVCAR3-FG This paper N/A Human ovarian cancer cell line OVCAR3-FGMSLN-/This paper N/A Human ovarian cancer cell line OVCAR8-FG This paper N/A Human ovarian cancer cell line SKOV3-FG This paper N/A Human artificial APC cell line This paper N/A Experimental models: Organisms/strains NOD.Cg-Prkdcscid Il2rgtm1Wjl/SzJ (NSG) The Jackson Laboratory Stock #: 005557 Oligonucleotides gRNA (MSLN): GCCAACGGCTCGAC CCCTGT Synthego N/A Recombinant DNA Vector: parental lentivector pMNDW Giannoni et al.67; Lan et al.171 N/A Software and algorithms FlowJo Software FlowJo https://www.flowjo.com/solutions/flowjo/ downloads AURA imaging software Spectral Instruments Imaging https://spectralinvivo.com/software/ ImageJ ImageJ https://imagej.net/Downloads Prism 9 Graphpad https://www.graphpad.com/scientific- software/prism/ Matlab The MathWorks, Inc http://www.mathworks.com/products/ matlab.html R R http://www.R-project.org/ RStudio RStudio https://posit.co e5 Med 6, 100804, October 10, 2025
Transfection:Article Title: Overcoming ovarian cancer resistance and evasion to CAR-T cell therapy by harnessing allogeneic CAR-NKT cells.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Lenti/FG This paper N/A Lenti/CD1d This paper N/A Lenti/MSLN This paper N/A Lenti/STAT1C-EGFP This paper N/A Biological samples Human peripheral blood mononuclear cells (PBMCs) UCLA N/A Primary ovarian cancer (OC) patient samples UCLA N/A Cord Blood CD34+ Stem/Progenitor Cells HemaCare (Charles River) CAT#CB34C-3 Chemicals, peptides, and recombinant proteins Streptavidin-HRP conjugate Invitrogen CAT#SA10001 IFN-γ (ELISA, standard) eBioscience CAT#29-8319-65 TNFα (ELISA, standard) eBioscience CAT#29-8329-65 IL-2 (ELISA, standard) eBioscience CAT#29-8029-65 IL-4 (ELISA, standard) eBioscience CAT#39-8049-65 IL-17A (ELISA, standard) eBioscience CAT#29-8179-65 Tetramethylbenzidine (TMB) KPL CAT#5120-0053 Ganciclovir (GCV) Sigma CAT#G2536 α-Galactosylceramide (KRN7000) Avanti Polar Lipids SKU#867000P-1mg Recombinant human IL-2 Peprotech CAT#200-02 Recombinant human IL-3 Peprotech CAT#200-03 Recombinant human IL-4 Peprotech CAT#200-04 Recombinant human IL-7 Peprotech CAT#200-07 Recombinant human IL-13 Peprotech CAT#200-13 Recombinant human IL-15 Peprotech CAT#200-15 Recombinant human IFN-γ Peprotech CAT#300-02 Recombinant human Flt3-Ligand Peprotech CAT#300-19 Recombinant human SCF Peprotech CAT#300-07 Recombinant human TPO Peprotech CAT#300-18 Recombinant human GM-CSF Peprotech CAT#300-03 Recombinant human M-CSF Peprotech CAT#300-25 L-ascorbic acid 2-phosphate Sigma CAT#A8960-5G B27TM Supplement (50X), serum free ThermoFisher CAT#17504044 Cas9-NLS purified protein UC Berkeley N/A X-VIVO 15 Serum-free Hematopoietic Cell Medium Lonza CAT#04-418Q RPMI1640 cell culture medium Corning Cellgro CAT#10-040-CV DMEM cell culture medium Corning Cellgro CAT#10-013-CV Fetal Bovine Serum (FBS) Sigma CAT#F2442 MACS BSA stock solution Miltenyi CAT#130-091-376 30% BSA Gemini CAT#700-110-100 Penicillin-StreptomycineGlutamine (P/S/G) Gibco CAT#10378016 Penicillin: streptomycin (pen:strep) solution (P/S) Gemini Bio-products CAT#400-109-100 MEM non-essential amino acids (NEAA) Gibco CAT#11140050 HEPES Buffer Solution Gibco CAT#15630080 Sodium Pyruvate Gibco CAT#11360070 Beta-Mercaptoethanol Sigma SKU#M6250 (Continued on next page) e3 Med 6, 100804, October 10, 2025 .. REAGENT or RESOURCE SOURCE IDENTIFIER Normocin Invivogen CATant-nr-2 Fixable Viability Dye eFluor506 eBioscience CAT#65-0866-14 Cell Fixation/Permeabilization Kit BD Biosciences CAT#554714 RetroNectin recombination human fibronectin fragment, 2.5mg Takara CAT#T100B 10% neutral-buffered formalin Richard-Allan Scientific CAT#5705 D-Luciferin Potassium Salt Bioluminescent Substrate Revivi CAT#122799-100MG Fluriso (Isoflurane) Vet One CAT#502017 Phosphate Buffered Saline (PBS) pH 7.4 (1X) Gibco CAT#10010-023 Formaldehyde Sigma-Aldrich CAT#F8775 Golgistop Protein Transport Inhibitor BD Biosciences CAT#554724 Poloxamer Synperonic F108 Millipore Sigma CAT#07579-250G-F Prostaglandin E2 (PGE2) Cayman Chemical CAT#14010 TrypLETM Express Enzyme ThermoFisher Scientific CAT#12605010 Phorbol-12-myristate-13-acetate (PMA) Sigma CAT#524400 Ionomycin, Calcium salt, Streptomyces conglobatus Sigma CAT#407952 Critical commercial assays Human NK Cell Isolation Kit Miltenyi Biotec CAT#130-092-657 Human CD34 MicroBeads Kit Miltenyi Biotec CAT#130-046-703 Human CD14 MicroBeads Kit Miltenyi Biotec CAT#130-050-201 Human Anti-NKT MicroBeads Miltenyi Biotec CAT#130-094-842 Human Anti-HLA-DR MicroBeads Miltenyi Biotec CAT#130-046-101 Human tumor cell isolation kit Miltenyi Biotec CAT#130-108-339 Fixation/Permeabilization Solution Kit BD Sciences CAT#55474 AmaxaTM P3 Primary Cell 4DNucleofectorTM X Kit S Lonza CAT#V4XP-3032 Dynabeads Human T-Activator CD3/CD28 ThermoFisher CAT#11131D miRNeasy Mini Kit Qiagen CAT#217004 Chromium single cell V(D)J enrichment kit, human T cell 10 x Genomics CAT#1000005 Cryostor cell cryopreservation media Sigma CAT#C2874-100ML StemSpan"" Lymphoid Differentiation Coating Material (100X) Stem Cell Technologies CAT#9925 StemSpan" SFEM II Stem Cell Technologies CAT#9605 ImmunoCult" Human CD3/CD28/CD2 T Cell Activator Stem Cell Technologies CAT#10970 TransIT-Lenti Transfection Reagent Mirus Bio CAT#MIR 6600 Amicon Ultra-15 Centrifugal Filter Unit Sigma CAT#UFC910024 Nunclon Sphera-Treated 96-Well Microplate ThermoFisher CAT#174929 CellQART 6-Well Cell Culture Insert Millicell CAT#9301002 Human IL-17A ELISA MAX Deluxe Kit Biolegend CAT#433915 Deposited data scRNA-seq for the primary OC patient sample profiling study This paper GEO: GSE272764 scRNA-seq for the in vivo therapeutic cell profiling study This paper GEO: GSE270820 (Continued on next page) Med 6, 100804, October 10, 2025 e4 .. REAGENT or RESOURCE SOURCE IDENTIFIER scRNA-seq for the in vivo tumor cell profiling study This paper GEO: GSE255431 scRNA-seq for the in vivo TME profiling study This paper GEO: GSE268749 scTCR-seq This paper GEO: GSE254262 Bulk RNA-seq (dataset 1) This paper GEO: GSE252379 Bulk RNA-seq (dataset 2) This paper GEO: GSE164425 Methyl-seq This paper GEO: GSE251909 scRNA-seq for primary OC patient sample profiling study (public dataset B) Zhang et al.50 GEO: GSE165897 scRNA-seq for primary OC patient sample profiling study (public dataset C) Xu et al.49 GEO: GSE184800 scRNA-seq for primary OC patient sample profiling study (public dataset D) Zheng et al.36 GSA-Human, PRJCA005422 Experimental models: Cell lines Human multiple myeloma (MM) cell line MM.1S ATCC CRL-2974 Human chronic myelogenous leukemia cancer cell line K562 ATCC CCL-243 Human ovarian cancer cell line OVCAR3 ATCC HTB-161 Human ovarian cancer cell line OVCAR8 NIH N/A Human ovarian cancer cell line SKOV3 ATCC HTB-77 Human multiple myeloma (MM) cell line MM.1S-FG This paper N/A Human multiple myeloma (MM) cell line MM.1S-MSLN-FG This paper N/A Human ovarian cancer cell line OVCAR3-FG This paper N/A Human ovarian cancer cell line OVCAR3-FGMSLN-/This paper N/A Human ovarian cancer cell line OVCAR8-FG This paper N/A Human ovarian cancer cell line SKOV3-FG This paper N/A Human artificial APC cell line This paper N/A Experimental models: Organisms/strains NOD.Cg-Prkdcscid Il2rgtm1Wjl/SzJ (NSG) The Jackson Laboratory Stock #: 005557 Oligonucleotides gRNA (MSLN): GCCAACGGCTCGAC CCCTGT Synthego N/A Recombinant DNA Vector: parental lentivector pMNDW Giannoni et al.67; Lan et al.171 N/A Software and algorithms FlowJo Software FlowJo https://www.flowjo.com/solutions/flowjo/ downloads AURA imaging software Spectral Instruments Imaging https://spectralinvivo.com/software/ ImageJ ImageJ https://imagej.net/Downloads Prism 9 Graphpad https://www.graphpad.com/scientific- software/prism/ Matlab The MathWorks, Inc http://www.mathworks.com/products/ matlab.html R R http://www.R-project.org/ RStudio RStudio https://posit.co e5 Med 6, 100804, October 10, 2025
Cell Culture:Article Title: Overcoming ovarian cancer resistance and evasion to CAR-T cell therapy by harnessing allogeneic CAR-NKT cells.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Lenti/FG This paper N/A Lenti/CD1d This paper N/A Lenti/MSLN This paper N/A Lenti/STAT1C-EGFP This paper N/A Biological samples Human peripheral blood mononuclear cells (PBMCs) UCLA N/A Primary ovarian cancer (OC) patient samples UCLA N/A Cord Blood CD34+ Stem/Progenitor Cells HemaCare (Charles River) CAT#CB34C-3 Chemicals, peptides, and recombinant proteins Streptavidin-HRP conjugate Invitrogen CAT#SA10001 IFN-γ (ELISA, standard) eBioscience CAT#29-8319-65 TNFα (ELISA, standard) eBioscience CAT#29-8329-65 IL-2 (ELISA, standard) eBioscience CAT#29-8029-65 IL-4 (ELISA, standard) eBioscience CAT#39-8049-65 IL-17A (ELISA, standard) eBioscience CAT#29-8179-65 Tetramethylbenzidine (TMB) KPL CAT#5120-0053 Ganciclovir (GCV) Sigma CAT#G2536 α-Galactosylceramide (KRN7000) Avanti Polar Lipids SKU#867000P-1mg Recombinant human IL-2 Peprotech CAT#200-02 Recombinant human IL-3 Peprotech CAT#200-03 Recombinant human IL-4 Peprotech CAT#200-04 Recombinant human IL-7 Peprotech CAT#200-07 Recombinant human IL-13 Peprotech CAT#200-13 Recombinant human IL-15 Peprotech CAT#200-15 Recombinant human IFN-γ Peprotech CAT#300-02 Recombinant human Flt3-Ligand Peprotech CAT#300-19 Recombinant human SCF Peprotech CAT#300-07 Recombinant human TPO Peprotech CAT#300-18 Recombinant human GM-CSF Peprotech CAT#300-03 Recombinant human M-CSF Peprotech CAT#300-25 L-ascorbic acid 2-phosphate Sigma CAT#A8960-5G B27TM Supplement (50X), serum free ThermoFisher CAT#17504044 Cas9-NLS purified protein UC Berkeley N/A X-VIVO 15 Serum-free Hematopoietic Cell Medium Lonza CAT#04-418Q RPMI1640 cell culture medium Corning Cellgro CAT#10-040-CV DMEM cell culture medium Corning Cellgro CAT#10-013-CV Fetal Bovine Serum (FBS) Sigma CAT#F2442 MACS BSA stock solution Miltenyi CAT#130-091-376 30% BSA Gemini CAT#700-110-100 Penicillin-StreptomycineGlutamine (P/S/G) Gibco CAT#10378016 Penicillin: streptomycin (pen:strep) solution (P/S) Gemini Bio-products CAT#400-109-100 MEM non-essential amino acids (NEAA) Gibco CAT#11140050 HEPES Buffer Solution Gibco CAT#15630080 Sodium Pyruvate Gibco CAT#11360070 Beta-Mercaptoethanol Sigma SKU#M6250 (Continued on next page) e3 Med 6, 100804, October 10, 2025 .. REAGENT or RESOURCE SOURCE IDENTIFIER Normocin Invivogen CATant-nr-2 Fixable Viability Dye eFluor506 eBioscience CAT#65-0866-14 Cell Fixation/Permeabilization Kit BD Biosciences CAT#554714 RetroNectin recombination human fibronectin fragment, 2.5mg Takara CAT#T100B 10% neutral-buffered formalin Richard-Allan Scientific CAT#5705 D-Luciferin Potassium Salt Bioluminescent Substrate Revivi CAT#122799-100MG Fluriso (Isoflurane) Vet One CAT#502017 Phosphate Buffered Saline (PBS) pH 7.4 (1X) Gibco CAT#10010-023 Formaldehyde Sigma-Aldrich CAT#F8775 Golgistop Protein Transport Inhibitor BD Biosciences CAT#554724 Poloxamer Synperonic F108 Millipore Sigma CAT#07579-250G-F Prostaglandin E2 (PGE2) Cayman Chemical CAT#14010 TrypLETM Express Enzyme ThermoFisher Scientific CAT#12605010 Phorbol-12-myristate-13-acetate (PMA) Sigma CAT#524400 Ionomycin, Calcium salt, Streptomyces conglobatus Sigma CAT#407952 Critical commercial assays Human NK Cell Isolation Kit Miltenyi Biotec CAT#130-092-657 Human CD34 MicroBeads Kit Miltenyi Biotec CAT#130-046-703 Human CD14 MicroBeads Kit Miltenyi Biotec CAT#130-050-201 Human Anti-NKT MicroBeads Miltenyi Biotec CAT#130-094-842 Human Anti-HLA-DR MicroBeads Miltenyi Biotec CAT#130-046-101 Human tumor cell isolation kit Miltenyi Biotec CAT#130-108-339 Fixation/Permeabilization Solution Kit BD Sciences CAT#55474 AmaxaTM P3 Primary Cell 4DNucleofectorTM X Kit S Lonza CAT#V4XP-3032 Dynabeads Human T-Activator CD3/CD28 ThermoFisher CAT#11131D miRNeasy Mini Kit Qiagen CAT#217004 Chromium single cell V(D)J enrichment kit, human T cell 10 x Genomics CAT#1000005 Cryostor cell cryopreservation media Sigma CAT#C2874-100ML StemSpan"" Lymphoid Differentiation Coating Material (100X) Stem Cell Technologies CAT#9925 StemSpan" SFEM II Stem Cell Technologies CAT#9605 ImmunoCult" Human CD3/CD28/CD2 T Cell Activator Stem Cell Technologies CAT#10970 TransIT-Lenti Transfection Reagent Mirus Bio CAT#MIR 6600 Amicon Ultra-15 Centrifugal Filter Unit Sigma CAT#UFC910024 Nunclon Sphera-Treated 96-Well Microplate ThermoFisher CAT#174929 CellQART 6-Well Cell Culture Insert Millicell CAT#9301002 Human IL-17A ELISA MAX Deluxe Kit Biolegend CAT#433915 Deposited data scRNA-seq for the primary OC patient sample profiling study This paper GEO: GSE272764 scRNA-seq for the in vivo therapeutic cell profiling study This paper GEO: GSE270820 (Continued on next page) Med 6, 100804, October 10, 2025 e4 .. REAGENT or RESOURCE SOURCE IDENTIFIER scRNA-seq for the in vivo tumor cell profiling study This paper GEO: GSE255431 scRNA-seq for the in vivo TME profiling study This paper GEO: GSE268749 scTCR-seq This paper GEO: GSE254262 Bulk RNA-seq (dataset 1) This paper GEO: GSE252379 Bulk RNA-seq (dataset 2) This paper GEO: GSE164425 Methyl-seq This paper GEO: GSE251909 scRNA-seq for primary OC patient sample profiling study (public dataset B) Zhang et al.50 GEO: GSE165897 scRNA-seq for primary OC patient sample profiling study (public dataset C) Xu et al.49 GEO: GSE184800 scRNA-seq for primary OC patient sample profiling study (public dataset D) Zheng et al.36 GSA-Human, PRJCA005422 Experimental models: Cell lines Human multiple myeloma (MM) cell line MM.1S ATCC CRL-2974 Human chronic myelogenous leukemia cancer cell line K562 ATCC CCL-243 Human ovarian cancer cell line OVCAR3 ATCC HTB-161 Human ovarian cancer cell line OVCAR8 NIH N/A Human ovarian cancer cell line SKOV3 ATCC HTB-77 Human multiple myeloma (MM) cell line MM.1S-FG This paper N/A Human multiple myeloma (MM) cell line MM.1S-MSLN-FG This paper N/A Human ovarian cancer cell line OVCAR3-FG This paper N/A Human ovarian cancer cell line OVCAR3-FGMSLN-/This paper N/A Human ovarian cancer cell line OVCAR8-FG This paper N/A Human ovarian cancer cell line SKOV3-FG This paper N/A Human artificial APC cell line This paper N/A Experimental models: Organisms/strains NOD.Cg-Prkdcscid Il2rgtm1Wjl/SzJ (NSG) The Jackson Laboratory Stock #: 005557 Oligonucleotides gRNA (MSLN): GCCAACGGCTCGAC CCCTGT Synthego N/A Recombinant DNA Vector: parental lentivector pMNDW Giannoni et al.67; Lan et al.171 N/A Software and algorithms FlowJo Software FlowJo https://www.flowjo.com/solutions/flowjo/ downloads AURA imaging software Spectral Instruments Imaging https://spectralinvivo.com/software/ ImageJ ImageJ https://imagej.net/Downloads Prism 9 Graphpad https://www.graphpad.com/scientific- software/prism/ Matlab The MathWorks, Inc http://www.mathworks.com/products/ matlab.html R R http://www.R-project.org/ RStudio RStudio https://posit.co e5 Med 6, 100804, October 10, 2025
Enzyme-linked Immunosorbent Assay:Article Title: Overcoming ovarian cancer resistance and evasion to CAR-T cell therapy by harnessing allogeneic CAR-NKT cells.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Lenti/FG This paper N/A Lenti/CD1d This paper N/A Lenti/MSLN This paper N/A Lenti/STAT1C-EGFP This paper N/A Biological samples Human peripheral blood mononuclear cells (PBMCs) UCLA N/A Primary ovarian cancer (OC) patient samples UCLA N/A Cord Blood CD34+ Stem/Progenitor Cells HemaCare (Charles River) CAT#CB34C-3 Chemicals, peptides, and recombinant proteins Streptavidin-HRP conjugate Invitrogen CAT#SA10001 IFN-γ (ELISA, standard) eBioscience CAT#29-8319-65 TNFα (ELISA, standard) eBioscience CAT#29-8329-65 IL-2 (ELISA, standard) eBioscience CAT#29-8029-65 IL-4 (ELISA, standard) eBioscience CAT#39-8049-65 IL-17A (ELISA, standard) eBioscience CAT#29-8179-65 Tetramethylbenzidine (TMB) KPL CAT#5120-0053 Ganciclovir (GCV) Sigma CAT#G2536 α-Galactosylceramide (KRN7000) Avanti Polar Lipids SKU#867000P-1mg Recombinant human IL-2 Peprotech CAT#200-02 Recombinant human IL-3 Peprotech CAT#200-03 Recombinant human IL-4 Peprotech CAT#200-04 Recombinant human IL-7 Peprotech CAT#200-07 Recombinant human IL-13 Peprotech CAT#200-13 Recombinant human IL-15 Peprotech CAT#200-15 Recombinant human IFN-γ Peprotech CAT#300-02 Recombinant human Flt3-Ligand Peprotech CAT#300-19 Recombinant human SCF Peprotech CAT#300-07 Recombinant human TPO Peprotech CAT#300-18 Recombinant human GM-CSF Peprotech CAT#300-03 Recombinant human M-CSF Peprotech CAT#300-25 L-ascorbic acid 2-phosphate Sigma CAT#A8960-5G B27TM Supplement (50X), serum free ThermoFisher CAT#17504044 Cas9-NLS purified protein UC Berkeley N/A X-VIVO 15 Serum-free Hematopoietic Cell Medium Lonza CAT#04-418Q RPMI1640 cell culture medium Corning Cellgro CAT#10-040-CV DMEM cell culture medium Corning Cellgro CAT#10-013-CV Fetal Bovine Serum (FBS) Sigma CAT#F2442 MACS BSA stock solution Miltenyi CAT#130-091-376 30% BSA Gemini CAT#700-110-100 Penicillin-StreptomycineGlutamine (P/S/G) Gibco CAT#10378016 Penicillin: streptomycin (pen:strep) solution (P/S) Gemini Bio-products CAT#400-109-100 MEM non-essential amino acids (NEAA) Gibco CAT#11140050 HEPES Buffer Solution Gibco CAT#15630080 Sodium Pyruvate Gibco CAT#11360070 Beta-Mercaptoethanol Sigma SKU#M6250 (Continued on next page) e3 Med 6, 100804, October 10, 2025 .. REAGENT or RESOURCE SOURCE IDENTIFIER Normocin Invivogen CATant-nr-2 Fixable Viability Dye eFluor506 eBioscience CAT#65-0866-14 Cell Fixation/Permeabilization Kit BD Biosciences CAT#554714 RetroNectin recombination human fibronectin fragment, 2.5mg Takara CAT#T100B 10% neutral-buffered formalin Richard-Allan Scientific CAT#5705 D-Luciferin Potassium Salt Bioluminescent Substrate Revivi CAT#122799-100MG Fluriso (Isoflurane) Vet One CAT#502017 Phosphate Buffered Saline (PBS) pH 7.4 (1X) Gibco CAT#10010-023 Formaldehyde Sigma-Aldrich CAT#F8775 Golgistop Protein Transport Inhibitor BD Biosciences CAT#554724 Poloxamer Synperonic F108 Millipore Sigma CAT#07579-250G-F Prostaglandin E2 (PGE2) Cayman Chemical CAT#14010 TrypLETM Express Enzyme ThermoFisher Scientific CAT#12605010 Phorbol-12-myristate-13-acetate (PMA) Sigma CAT#524400 Ionomycin, Calcium salt, Streptomyces conglobatus Sigma CAT#407952 Critical commercial assays Human NK Cell Isolation Kit Miltenyi Biotec CAT#130-092-657 Human CD34 MicroBeads Kit Miltenyi Biotec CAT#130-046-703 Human CD14 MicroBeads Kit Miltenyi Biotec CAT#130-050-201 Human Anti-NKT MicroBeads Miltenyi Biotec CAT#130-094-842 Human Anti-HLA-DR MicroBeads Miltenyi Biotec CAT#130-046-101 Human tumor cell isolation kit Miltenyi Biotec CAT#130-108-339 Fixation/Permeabilization Solution Kit BD Sciences CAT#55474 AmaxaTM P3 Primary Cell 4DNucleofectorTM X Kit S Lonza CAT#V4XP-3032 Dynabeads Human T-Activator CD3/CD28 ThermoFisher CAT#11131D miRNeasy Mini Kit Qiagen CAT#217004 Chromium single cell V(D)J enrichment kit, human T cell 10 x Genomics CAT#1000005 Cryostor cell cryopreservation media Sigma CAT#C2874-100ML StemSpan"" Lymphoid Differentiation Coating Material (100X) Stem Cell Technologies CAT#9925 StemSpan" SFEM II Stem Cell Technologies CAT#9605 ImmunoCult" Human CD3/CD28/CD2 T Cell Activator Stem Cell Technologies CAT#10970 TransIT-Lenti Transfection Reagent Mirus Bio CAT#MIR 6600 Amicon Ultra-15 Centrifugal Filter Unit Sigma CAT#UFC910024 Nunclon Sphera-Treated 96-Well Microplate ThermoFisher CAT#174929 CellQART 6-Well Cell Culture Insert Millicell CAT#9301002 Human IL-17A ELISA MAX Deluxe Kit Biolegend CAT#433915 Deposited data scRNA-seq for the primary OC patient sample profiling study This paper GEO: GSE272764 scRNA-seq for the in vivo therapeutic cell profiling study This paper GEO: GSE270820 (Continued on next page) Med 6, 100804, October 10, 2025 e4 .. REAGENT or RESOURCE SOURCE IDENTIFIER scRNA-seq for the in vivo tumor cell profiling study This paper GEO: GSE255431 scRNA-seq for the in vivo TME profiling study This paper GEO: GSE268749 scTCR-seq This paper GEO: GSE254262 Bulk RNA-seq (dataset 1) This paper GEO: GSE252379 Bulk RNA-seq (dataset 2) This paper GEO: GSE164425 Methyl-seq This paper GEO: GSE251909 scRNA-seq for primary OC patient sample profiling study (public dataset B) Zhang et al.50 GEO: GSE165897 scRNA-seq for primary OC patient sample profiling study (public dataset C) Xu et al.49 GEO: GSE184800 scRNA-seq for primary OC patient sample profiling study (public dataset D) Zheng et al.36 GSA-Human, PRJCA005422 Experimental models: Cell lines Human multiple myeloma (MM) cell line MM.1S ATCC CRL-2974 Human chronic myelogenous leukemia cancer cell line K562 ATCC CCL-243 Human ovarian cancer cell line OVCAR3 ATCC HTB-161 Human ovarian cancer cell line OVCAR8 NIH N/A Human ovarian cancer cell line SKOV3 ATCC HTB-77 Human multiple myeloma (MM) cell line MM.1S-FG This paper N/A Human multiple myeloma (MM) cell line MM.1S-MSLN-FG This paper N/A Human ovarian cancer cell line OVCAR3-FG This paper N/A Human ovarian cancer cell line OVCAR3-FGMSLN-/This paper N/A Human ovarian cancer cell line OVCAR8-FG This paper N/A Human ovarian cancer cell line SKOV3-FG This paper N/A Human artificial APC cell line This paper N/A Experimental models: Organisms/strains NOD.Cg-Prkdcscid Il2rgtm1Wjl/SzJ (NSG) The Jackson Laboratory Stock #: 005557 Oligonucleotides gRNA (MSLN): GCCAACGGCTCGAC CCCTGT Synthego N/A Recombinant DNA Vector: parental lentivector pMNDW Giannoni et al.67; Lan et al.171 N/A Software and algorithms FlowJo Software FlowJo https://www.flowjo.com/solutions/flowjo/ downloads AURA imaging software Spectral Instruments Imaging https://spectralinvivo.com/software/ ImageJ ImageJ https://imagej.net/Downloads Prism 9 Graphpad https://www.graphpad.com/scientific- software/prism/ Matlab The MathWorks, Inc http://www.mathworks.com/products/ matlab.html R R http://www.R-project.org/ RStudio RStudio https://posit.co e5 Med 6, 100804, October 10, 2025
In Vivo:Article Title: Overcoming ovarian cancer resistance and evasion to CAR-T cell therapy by harnessing allogeneic CAR-NKT cells.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Lenti/FG This paper N/A Lenti/CD1d This paper N/A Lenti/MSLN This paper N/A Lenti/STAT1C-EGFP This paper N/A Biological samples Human peripheral blood mononuclear cells (PBMCs) UCLA N/A Primary ovarian cancer (OC) patient samples UCLA N/A Cord Blood CD34+ Stem/Progenitor Cells HemaCare (Charles River) CAT#CB34C-3 Chemicals, peptides, and recombinant proteins Streptavidin-HRP conjugate Invitrogen CAT#SA10001 IFN-γ (ELISA, standard) eBioscience CAT#29-8319-65 TNFα (ELISA, standard) eBioscience CAT#29-8329-65 IL-2 (ELISA, standard) eBioscience CAT#29-8029-65 IL-4 (ELISA, standard) eBioscience CAT#39-8049-65 IL-17A (ELISA, standard) eBioscience CAT#29-8179-65 Tetramethylbenzidine (TMB) KPL CAT#5120-0053 Ganciclovir (GCV) Sigma CAT#G2536 α-Galactosylceramide (KRN7000) Avanti Polar Lipids SKU#867000P-1mg Recombinant human IL-2 Peprotech CAT#200-02 Recombinant human IL-3 Peprotech CAT#200-03 Recombinant human IL-4 Peprotech CAT#200-04 Recombinant human IL-7 Peprotech CAT#200-07 Recombinant human IL-13 Peprotech CAT#200-13 Recombinant human IL-15 Peprotech CAT#200-15 Recombinant human IFN-γ Peprotech CAT#300-02 Recombinant human Flt3-Ligand Peprotech CAT#300-19 Recombinant human SCF Peprotech CAT#300-07 Recombinant human TPO Peprotech CAT#300-18 Recombinant human GM-CSF Peprotech CAT#300-03 Recombinant human M-CSF Peprotech CAT#300-25 L-ascorbic acid 2-phosphate Sigma CAT#A8960-5G B27TM Supplement (50X), serum free ThermoFisher CAT#17504044 Cas9-NLS purified protein UC Berkeley N/A X-VIVO 15 Serum-free Hematopoietic Cell Medium Lonza CAT#04-418Q RPMI1640 cell culture medium Corning Cellgro CAT#10-040-CV DMEM cell culture medium Corning Cellgro CAT#10-013-CV Fetal Bovine Serum (FBS) Sigma CAT#F2442 MACS BSA stock solution Miltenyi CAT#130-091-376 30% BSA Gemini CAT#700-110-100 Penicillin-StreptomycineGlutamine (P/S/G) Gibco CAT#10378016 Penicillin: streptomycin (pen:strep) solution (P/S) Gemini Bio-products CAT#400-109-100 MEM non-essential amino acids (NEAA) Gibco CAT#11140050 HEPES Buffer Solution Gibco CAT#15630080 Sodium Pyruvate Gibco CAT#11360070 Beta-Mercaptoethanol Sigma SKU#M6250 (Continued on next page) e3 Med 6, 100804, October 10, 2025 .. REAGENT or RESOURCE SOURCE IDENTIFIER Normocin Invivogen CATant-nr-2 Fixable Viability Dye eFluor506 eBioscience CAT#65-0866-14 Cell Fixation/Permeabilization Kit BD Biosciences CAT#554714 RetroNectin recombination human fibronectin fragment, 2.5mg Takara CAT#T100B 10% neutral-buffered formalin Richard-Allan Scientific CAT#5705 D-Luciferin Potassium Salt Bioluminescent Substrate Revivi CAT#122799-100MG Fluriso (Isoflurane) Vet One CAT#502017 Phosphate Buffered Saline (PBS) pH 7.4 (1X) Gibco CAT#10010-023 Formaldehyde Sigma-Aldrich CAT#F8775 Golgistop Protein Transport Inhibitor BD Biosciences CAT#554724 Poloxamer Synperonic F108 Millipore Sigma CAT#07579-250G-F Prostaglandin E2 (PGE2) Cayman Chemical CAT#14010 TrypLETM Express Enzyme ThermoFisher Scientific CAT#12605010 Phorbol-12-myristate-13-acetate (PMA) Sigma CAT#524400 Ionomycin, Calcium salt, Streptomyces conglobatus Sigma CAT#407952 Critical commercial assays Human NK Cell Isolation Kit Miltenyi Biotec CAT#130-092-657 Human CD34 MicroBeads Kit Miltenyi Biotec CAT#130-046-703 Human CD14 MicroBeads Kit Miltenyi Biotec CAT#130-050-201 Human Anti-NKT MicroBeads Miltenyi Biotec CAT#130-094-842 Human Anti-HLA-DR MicroBeads Miltenyi Biotec CAT#130-046-101 Human tumor cell isolation kit Miltenyi Biotec CAT#130-108-339 Fixation/Permeabilization Solution Kit BD Sciences CAT#55474 AmaxaTM P3 Primary Cell 4DNucleofectorTM X Kit S Lonza CAT#V4XP-3032 Dynabeads Human T-Activator CD3/CD28 ThermoFisher CAT#11131D miRNeasy Mini Kit Qiagen CAT#217004 Chromium single cell V(D)J enrichment kit, human T cell 10 x Genomics CAT#1000005 Cryostor cell cryopreservation media Sigma CAT#C2874-100ML StemSpan"" Lymphoid Differentiation Coating Material (100X) Stem Cell Technologies CAT#9925 StemSpan" SFEM II Stem Cell Technologies CAT#9605 ImmunoCult" Human CD3/CD28/CD2 T Cell Activator Stem Cell Technologies CAT#10970 TransIT-Lenti Transfection Reagent Mirus Bio CAT#MIR 6600 Amicon Ultra-15 Centrifugal Filter Unit Sigma CAT#UFC910024 Nunclon Sphera-Treated 96-Well Microplate ThermoFisher CAT#174929 CellQART 6-Well Cell Culture Insert Millicell CAT#9301002 Human IL-17A ELISA MAX Deluxe Kit Biolegend CAT#433915 Deposited data scRNA-seq for the primary OC patient sample profiling study This paper GEO: GSE272764 scRNA-seq for the in vivo therapeutic cell profiling study This paper GEO: GSE270820 (Continued on next page) Med 6, 100804, October 10, 2025 e4 .. REAGENT or RESOURCE SOURCE IDENTIFIER scRNA-seq for the in vivo tumor cell profiling study This paper GEO: GSE255431 scRNA-seq for the in vivo TME profiling study This paper GEO: GSE268749 scTCR-seq This paper GEO: GSE254262 Bulk RNA-seq (dataset 1) This paper GEO: GSE252379 Bulk RNA-seq (dataset 2) This paper GEO: GSE164425 Methyl-seq This paper GEO: GSE251909 scRNA-seq for primary OC patient sample profiling study (public dataset B) Zhang et al.50 GEO: GSE165897 scRNA-seq for primary OC patient sample profiling study (public dataset C) Xu et al.49 GEO: GSE184800 scRNA-seq for primary OC patient sample profiling study (public dataset D) Zheng et al.36 GSA-Human, PRJCA005422 Experimental models: Cell lines Human multiple myeloma (MM) cell line MM.1S ATCC CRL-2974 Human chronic myelogenous leukemia cancer cell line K562 ATCC CCL-243 Human ovarian cancer cell line OVCAR3 ATCC HTB-161 Human ovarian cancer cell line OVCAR8 NIH N/A Human ovarian cancer cell line SKOV3 ATCC HTB-77 Human multiple myeloma (MM) cell line MM.1S-FG This paper N/A Human multiple myeloma (MM) cell line MM.1S-MSLN-FG This paper N/A Human ovarian cancer cell line OVCAR3-FG This paper N/A Human ovarian cancer cell line OVCAR3-FGMSLN-/This paper N/A Human ovarian cancer cell line OVCAR8-FG This paper N/A Human ovarian cancer cell line SKOV3-FG This paper N/A Human artificial APC cell line This paper N/A Experimental models: Organisms/strains NOD.Cg-Prkdcscid Il2rgtm1Wjl/SzJ (NSG) The Jackson Laboratory Stock #: 005557 Oligonucleotides gRNA (MSLN): GCCAACGGCTCGAC CCCTGT Synthego N/A Recombinant DNA Vector: parental lentivector pMNDW Giannoni et al.67; Lan et al.171 N/A Software and algorithms FlowJo Software FlowJo https://www.flowjo.com/solutions/flowjo/ downloads AURA imaging software Spectral Instruments Imaging https://spectralinvivo.com/software/ ImageJ ImageJ https://imagej.net/Downloads Prism 9 Graphpad https://www.graphpad.com/scientific- software/prism/ Matlab The MathWorks, Inc http://www.mathworks.com/products/ matlab.html R R http://www.R-project.org/ RStudio RStudio https://posit.co e5 Med 6, 100804, October 10, 2025
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